IL－4对多发性骨髓瘤患者外周血细胞CD20及HLA－DR表达的影响

EFFECTS OF INTERLEUKIN 4 ON THE PHENOTYPES RELATED TO ANTIBODY PRODUCTON OF PERIPHERAL BLOOD CELLS IN PATIENTS WITH MULTIPLE MYELOMA

本文报道用流式细胞分析技术，检测了２８例多发性骨髓瘤（ＭＭ）患者和２０名健康对照者外周血Ｂ细胞（ＣＤ２０＋）以及Ｂ、Ｔ和单核细胞中ＨＬＡ－ＤＲ＋细胞比率。结果发现，ＭＭ患者ＣＤ２０＋以及Ｂ、Ｔ及单核细胞中ＨＬＡ－ＤＲ＋细胞比率与正常对照组差异非常显著（Ｐ＜０．０１）。加入重组ＩＬ－４，可使８名ＭＭ患者ＣＤ２０＋、ＨＬＡ－ＤＲ＋ＣＤ２０＋、ＨＬＡ－ＤＲ＋单核细胞比率提高非常显著（Ｐ＜０．０１）。而在Ｔ细胞，Ｐ值则＞０．０５。我们认为由于ＩＬ－４分泌减少，一方面使多克隆Ｂ细胞激活及增殖受抑，另一方面使单核细胞ＨＬＡ－ＤＲ抗原表达减少，抗原提呈能力下降可能是ＭＭ发生多克隆免疫球蛋白抑制的重要原因。

The phenotypes of peripheral blood cells of 28 patients with multiple myeloma(MM)and of 20 normal con-trols were analysed with indirect immunofluorescent staining and flow cytometry.It was found that the percent-ages of CD20+cells in PBMCs and HLA-DR+cells in B cells,T cells and monocytes were.38± 83%,26. 04±6.80%,12.50±4.22%and 33.63±16.24%in MM vs 12.20±2.92%,34.30±5.20%,14.30±3.36%and 61.45±10.10%in normal controls(P<0.01 ),respectively. CD20+ cells among PMBCs,HLA-DR+ cells among B andmonocytes significantly increased when adding rhIL-4 100U /ml in peripheral blood of 8 patients with MM for 12 h(P <0. 01 ).Our results indicated that suppression of B lymphocyte activation and decreased antigen prescntationcapability of monocytes,which might be due to IL-4 production,mainly accounted for reduced polyclonal im-munoglobulin synthesis.