HIT^＋4细胞的分布及增殖特性的研究

DISTRIBUTION AND PROLIFERATIVE PROPERTIES OF HIT4+ CELLS

观察了ＨＩＴ４＋细胞在正常人外周血ＣＤ４和ＣＤ８亚群中的分布及ＣＤ４＋ＨＩＴ４＋、ＣＤ４＋ＨＩＴ４－细胞的增殖特性。结果发现：１．ＨＩＴ４＋细胞在ＣＤ４和ＣＤ８亚群所占的百分率分别为３６．９±６．６％和１９．７±４．８％。２．ＣＤ４＋ＨＩＴ４＋细胞对ＣｏｎＡ、ＴＴ、ＰＰＤ和Ａｕｔｏ－Ｂ刺激的增殖反应明显低于ＣＤ４＋ＨＩＴ４－细胞，而后者与总ＣＤ４亚群的增殖反应水平接近。将ＨＩＴ４＋细胞和ＨＩＴ４－细胞的分布与增殖特性与已报道的Ｔ亚－亚群细胞比较，提示ＨＩＴ４可能为识别Ｔ亚－亚群的一个新标志。

The distribution of HIT4+ cells between CD4 and CD8 subsets and the proliferative properties of CD4+ HIT4+cells and CD4+HIT4-cells were investigated. We demonstrated by two-color fluorescence stain that HIT4 McAbwas reactive with 36.9±6.6%of CD4 subset and 19.7±4.8%of CD8 subset.HIT 4 McAb could subdivide pe-ripheral blood CD4 and CD8 subsets into CD4+ HIT4+ ,CD4+ HIT4-,CD8+ HIT4+ and CD8+ HIT4-subpopulation-subsets respectively.CD4+HIT4+ cells proliferated poorly upon stimulus of ConA ,TT ,PPD or mitomycin-C treatedautologous B cells. The CD4+ HIT4-cells ,in contrast,proliferated well to all of those stimulators. Comparison ofthe distribution and proliferative properties of HIT4+ cells and HIT4-cells with other known T subpopulation-sub-set cells indicated that HIT4 might be a new marker of T subpopulation-subset.