小鼠胸腺树突状细胞系MTSC4诱导早期T细胞株C320表达IL－2R

MOUSE THYMUS DENDRITEC CELL LINE MTSC4 INDUCE IN VITRO PRE-T LYMPHOCYTE CLONE C320 CELLS EXPESSING IL-2R

通过流式细胞仪（ＦＡＣＳ）分析了我室自建的小鼠胸腺树突状细胞系ＭＴＳＣ４与小鼠肿瘤性早期Ｔ细胞株Ｃ３２０细胞共育后，Ｃ３２０细胞表达ＩＬ－２Ｒ的状态，分析了ＣｏｎＡ和ＰＨＡ对Ｃ３２０及与ＭＴＳＣ４共育后的Ｃ３２０表达ＩＬ－２Ｒ的诱导促进作用以及ＩＬ－２Ｒ＋Ｃ３２０细胞在脱离外源性刺激后其比例的变化。ＭＴＳＣ４及ＣｏｎＡ和ＰＨＡ在不同程度上抑制Ｃ３２０细胞的无限制增殖，ＣｏｎＡ和ＰＨＡ对与ＭＴＳＣ４共育后的Ｃ３２０细胞的抑制增殖作用更强。

The CD25 (IL-2R ,P55)-pgp-1+CD4-CD8- CD3 LFA-1+ mouse Pre-T lymphocyte clone C320 could be in-duced to express IL-2R by co-culture with mouse thymic dendritic cell line MTSC4. The fraction of C320 cell re-covered after 7 days and 14 days from these cultures could express IL-2R analysed by FACS. The observation indi-cated that these in vitro gencrated IL-2R+C320 cells were responsive to Con A and PHA to express more IL-2R.Cell surface phenotyping suggests that IL-2R + pre-T cells are more mature than IL-2R-pte-T cell. The rate ofIL-2R+ cell was reduced without any exogenous stimulants.MTSC4,Con A and PHA could inhibit unlimited pro-liferation of C320 calls in different degrees.