碱性髓鞘蛋白反应的CD^（4＋）细胞及被动转移诱导实验性变态反应性脑脊髓炎

MYELIN BASIC PROTEIN REACTIVE CK4+T CELL LINE AND ADOPTIVE TRANSFER OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

用碱性髓鞘蛋白加福氏完全佐剂免疫ＳＪＬ／Ｊ小鼠，建立实验性变态反应性脑脊髓炎动物模型。取发病动物淋巴结作细胞培养，并用碱性髓鞘蛋白激活培养３周。其间２次经Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ分离纯化激活的细胞，并加Ｘ线照射的脾细胞，应用３Ｈ－ＴｄＲ掺入法测定细胞对碱性髓鞘蛋白的反应状况。细胞经ＰＫＨ２－ＧＬ标记及ＰＥ－抗ＣＤ４荧光染色，流式细胞仪分析细胞阳性情况。ＰＫＨ２－ＧＬ标记的细胞静脉注入同品系健康小鼠，约１０天后，动物发生典型的实验性变态反应性脑脊髓炎症状。随之分离发病动物的中枢神经系统和淋巴结淋巴细胞，发现于中枢神经系统中ＰＫＨ２－ＧＬ标记细胞中４５％为ＣＤ４＋细胞，其中９０％以上细胞为激活的母细胞。而在淋巴结中ＣＤ４＋细胞低于４％，母细胞少于１０％，结果说明对碱性髓鞘蛋白反应的ＣＤ４＋细胞，可选择性地进入中枢神经系统并介导实验性变态反应性脑脊髓炎的免疫病理反应。

ExperIMental allergic encephalomyelitis (EA E ) was established in SJL/J mice inoculated with complete Fre-und's adjuvant conjugated myelin basic protein(MBP ).The lymph-nodes of the morbid mice were taken,of whichthe lymphocytes were cultured.The cultured cells were activated with MBP and a cell strain of CD4 that wasspecifically reactivc to MBP was set up.The cells labelled with PKH 2-GL were inoculated intravenously intohealthy mice of the same strain.And the passive transfer of EAE was successfully established.Therefore,it sugeststhat the CD4 cells may be directly responsible for the induction of EAE.