胸腺因子D对LAK细胞活性诱导的影响

SYNERGETIC EFFECT OF ITYMIC FACTOR D AND RECOMBINANT IL-2ON THE GENERATION OF LAK CELL CYTOTOXICITY

本文报道利用ＭＴＴ法和４小时５１Ｃｒ释放法，在有或无ＩＬ－２存在的情况下，研究了ＴＦＤ对正常人ＰＢＭＣ体外增殖、ＬＡＫ活性诱导的影响。结果表明：单纯ＴＦＤ不能促进静止的淋巴细胞增殖，也不能诱导出高活性的ＬＡＫ细胞，但可促使经ＩＬ－２活化的淋巴细胞进一步增殖如先用ＩＬ－２活化淋巴细胞２４小时，再加入ＴＦＤ联合诱导，第４天时，ＬＡＫ活性可达８１．３±６．５％，明显高于单用ＩＬ－２组（Ｐ＜０．０１）。

The regulatory effect of thymic factor D(TPD)on the proliferation and induction of IL-2-mediated LAKwasinvestigated with MTT method and 4-hour 51Cr release assay. The results showed that the proliferative reaction oflymphocytes after the culture treated with IL-2and TFD was significantly enhanced as compared with that of lym-phocytes cultured with rIL-2alone(P<0.01).A lthough TFD alone was not capable of inducing LAK activity,lymphocytes preincubated with rIL-2( 1 00U/ml ) for 24 hours,and then added with T FD for 72 hours showed a sig-nificantly enhanced L AK cytotoxicity as compared with that of control group(P<0.01).These results suggest thatTFDmay enhance the IL-2-mediated induction of LAK activity in vitro.