PCR快速检测幽门螺杆菌

RAPID DETECTION OF HELICOBACTER PYLORI WITH THE POLYMERASW CHAIN REACTION

应用ＰＣＲ扩增１５个幽门螺杆菌分离株的ＤＮＡ，扩增产物经琼脂糖凝胶电泳均显示一条２９８ｂｐ的区带，而１２株与幽门螺杆菌相关的肠道杆菌都不能扩增出该片段。ＰＣＲ可检出少至１００个幽门螺杆菌细胞，并能检出胃粘膜活检标本中的此菌，全部实验可在５小时内完成。

A polymerase chain reaction(PCR )assay for the specific detection of H elicobacter pylori was developed by us-ing primers based on the nucleotide sequences of a species-specific antigen of H. pylori.The PCR assay amplified a298-nucleotide-pair product which was analyzed by agarose gel electrophoresis.PCR detected all 15 H. pylori iso-lates tested,and did not amplify sequences in several closely related species.The PCR test was able to detect asfew as 100 H.pylori cells.H. pylori in fresh gastric biopsy specimens was detected successfully in five hours byPCR. Therefore PCR is a rapid,sensitive and specific method for the detection of H.pylori.