宋内氏志贺氏菌（Ipa^＋,Oag^—）菌株的构建及表达

CLONING OF INVASIVE PLASMID ANTIGEN GENES FROM SHIGELLA SPP.AND CONSTRUCTION OF SHIGELLA SONNEL(Lpa+,Oag-)STRAINS

直接用转化方法将质粒ｐＨＳ４１０８转入宋内氏Ⅱ相菌Ｓ１Ｒ（ｌｐａ－，Ｏａｇ－），构建了菌株Ｓ１Ｒ１０１／ｐＨＳ４１０８。该菌株能表达侵袭性外膜多肽ａ，ｂ，ｃ，ｄ，无Ｏ抗原。本实验又将质粒ｐＨＳ４１０８中的１２．５ｋｂＳａｌⅠ片段克隆到质粒ｐＢＲ３２２上，构建质粒ｐＪＭ５６，并把它转入Ｓ１Ｒ株内所得的重组菌Ｓ１Ｒ１０２／ｐＪＭ５６能表达多肽ａ，ｃ，ｄ，但不表达多肽ｂ。

y direct transforming the plasmid pHS4108 into a recipient strain,Shigella sonnei form IIS1R(Ipa-,Oag- ),we constructed a recombinant strain S1 R101/pHS4108 in this study. It was shown that S1R101/pHS4108 was ableto express polypeptides a,b.c,and d but no O antigen. By fruther subcloning a 12.5kb SalⅠ DNA fragmentfrom pHS4108 into plasmld pBR322.we also constructed a plasmid pJM56.This plasmid was then transformed in-to Shigella sonnei strain S1R,thus a recombinant Strain S1R 102/pJM56 was obtained.S1R 102/pJM56 was capableof expressing polypeptides a.C. d except b.