摘要
用重组基因技术将产肠毒素大肠杆菌（ＥＴＥＣ）的耐热肠毒素（ＳＴａ）基因与不耐热肠毒素Ｂ亚单位（ＬＴ－Ｂ）基因融合。表达的ＬＴＢ／ＳＴａ融合蛋白，既有ＬＴ－Ｂ的抗原性又有ＳＴａ的抗原性。由于基因融合的方式不同，对ＳＴａ的抗原性影响很大，但对ＬＴ－Ｂ的抗原性没有影响。ＬＴ－Ｂ／ＳＴａ融合多肽保留了ＥＴＥｃ肠毒素结合ＧＭ－１神经节甙酯的能力，却仍具有ＳＴａ的生物毒性。
he fusion
genes,comprising the genes that code for the B subunit of the heat-labile
enterotoxin(LT-B)andthe heat-stable enterotoxin(STa)of enterotoxigenic E scherichia coli(ETEC).
have been constructed by recombi-nant genetic techniques.The LT-B/STa fusion peptides were
not only of ST antigenicity but also of LT-B anti-genicity. For the different constructions,the
expression levels of ST antigenicity differred greatly , but LT anti-genicity were similar.The
fusion proteins retained the ability to bind to GM-1 ganglioside and native toxicity ofSTa.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第4期219-222,共4页
Chinese Journal of Microbiology and Immunology
基金
国家"八六三"计划资助
关键词
产肠毒素
肠毒素
基因融合
Enterotoxigenic Escherichia coli(ETEC)
Enterotoxin
Gene fusion
