DCX的制备及其对肿瘤细胞的体外杀伤作用

THE EXTRACTION OF DCX AND ITS ITS CYTOTOXIC ACTIVITY TO TUMOR CELLS IN VITRO

本文报道粘质沙雷氏菌细胞壁含有大量ＤＣＸ成分，用多种有机溶剂反复抽提可使ＤＣＸ产率达８．５％。体外细胞毒实验发现微量ＤＣＸ（４ηｇ／ｍｌ）３～４ｈ即可直接杀伤ＳＭＭＣ－７７２１细胞株、ＨｅＬａ细胞株、ｋＭ３细胞株和Ｓ０肉瘤细胞株，而对纤维母细胞３Ｔ３株和大鼠肝细胞等则无明显的影响，表明ＤＣＸ能够直接杀伤体外培养的肿瘤细胞，是一种能够选择性地杀伤肿瘤细胞的生物制剂。

he cell wall of Serratia marcescens contains a Iarge amount of DCX ,Treated with a mixture of different or-ganic solvents.the bacteria could yield DCX up to 8.5%,The cytotoxicity assay showed that a small amount ofDCX(2-4 ng/ml)could kill SMMC-7721,HeLa,KM3 and So strain cells rapidly(3-4h),but DCX did not have obvi-ous effect on 3T3 strain cells and murine hepatocytes.The result meant that DCX could kill tumor cells directly andwas a selective anti-tumor preparation.