恙虫病立克次体sta58主要抗原基因片段的克隆及表达

MOLECULAR CLONING AND EXPRESSION OF PARPIAL sta58 MAJOR ANTIGEN GENE OF RICKETTSIA TSUTSUGAMUSHI IN ESCHERICHIA COLI

作者设计合成一对ＤＮＡ引物，经ＰＣＲ扩增出Ｋａｒｐ及ＣＭＹ株恙虫病立克次体ｓｔａ５８主要抗原基因片段，分别建立其无性繁殖系，构建了表达质粒ｐＢＶＲＫ５和ｐＢＶＲＣ４５，在国内首次应用大肠杆菌成功表达了恙虫病立克次体抗原。其意义在于为我国恙虫病立克次体研究提供特异性核酸探针，为恙虫病基因工程诊断试剂的研制奠定物质基础。

e present here the first application of recombinant DNA technology and PCR technology in the research of rickettsiae in this country.A 987-base pair fragment of Sta58 major antigen gene of R.tsutsugamushi was ampli-fied,by using PCR technology,cloned and expressed in E.coli.The cloned rickettsial gene could be used as specificB.tsutsugamushi DNA probe,and the expressed rickettsial polypeptide antigen could be a candidate diagnosticreagent for scrub typhus.