何杰金氏淋巴瘤细胞的流式细胞和AgNORs计数分析

Analysis of Flow Cytometry and AgNORs in Hodgkin's Lymphomas

应用流式细胞术和ＡｇＮＯＲｓ计数对何杰金氏淋巴瘤细胞的ＤＮＡ指数、细胞增殖周期和核仁组成区等资料分析。癌细胞的ＤＮＡ指数为０．９７～１．４９，均值为１．２０±０．１５；非整倍体率为７７．５％（３１／４０例），以超二倍体为主；％Ｓ和ＰＩ分别为１７．５６％和２８．９６％。ＡｇＮＯＲｓ计数为２．９９～８．３４／核，均值为５．８９±０．８８。各组织学类型的ＤＮＡ指数和％Ｓ值之间无明显差别。ＡｇＮＯＲｓ计数与％Ｓ有关，而与代表着倍体的ＤＮＡ指数无关。

Forty cases of Hodgkin's Lymphomas were analysed by flow cytometry and argyrophilic staining(AgNOR) technique for the data of DNA Index(DI), proliferation cycle and enumeration of AgNORs foci. The results showed that ①DI valuer ranged from 0.97 - 1. 49 with an average of 1.20.② Aneuploid frequency was 77.5% (31/40 cases), the DNA hyperdiploidy was occupied a dominant position. ③The percentage of S phase cells (%S) and PI were 17.56% and 28. 96% respectively.④The enumeration of AgNORs was 2. 99- 8.34 per nucleus, with an average of 5. 89. There was no significant correlation for the DI and %S among th histopathologic subtypes. It was suggested that the AgNORs count might be related to the %S rather than to DI,representing DNA aneuploidy. Utilizing analysis of cytometry and AgNORs data, it may offer an objective basis for futher studying heterogeneity in this disorder.