食品中苏丹红1号的GC-MS/SIM快速分析方法研究

Rapid Determination of Sudan Red1in Food by GC-MS/SIM

采用气相色谱-质谱(GC-MS)选择离子检测法(SIM),提出一种测定食品中苏丹红1号的新方法。色谱柱30m×0.25mmPR-SR石英毛细管柱,进样口温度280℃,柱温200℃,10℃/min升至280℃;柱前压100kPa,载气He;EI离子源,选择m/z77、115、143、248离子用于SIM检测,并根据这4个抽出离子的峰面积比进行确证。苏丹红1号的线性范围为0.01～10.0mg/L,相对标准偏差小于6.1%,回收率85%～90%,检出限为0.001mg/kg,每个样品分析时间为5min。本法与欧洲健康与消费者保护委员会发布的方法(HPLC法)相比灵敏度高两个数量级,分析时间缩短,用色谱保留时间、质谱同时定性,消除了食品中杂质的干扰,避免了只用色谱保留时间定性可能产生的假阳性,结果准确可靠,选择性和重复性好,适用于所有食品成品及原料的检验。

A newmethod was established for determining Sudan Red1in food by gas chromatography-mass spectrumwithselected ionmonitor(GC-MS/SIM).The conditions ofGC-MSwere optimized,andthe selectˉed ions were m/z77,115,143and248,respectively.The interferences caused by the impurities in food could be avoided bythis method.The linear range of the determination was0.01-10.0mg/Lwith a determiˉnation limit of0.001mg/kg.The recovery range was85%-90%and the RSD was less than6.1%.The method has been proved to be reliable and accurate.It has been applied to the determination of Sudan Red1in food with satisfactory results.