青年和老年人大肠癌DNA含量的流式细胞分析

DNA Content Analysis of the Colorectal Cancer in the Elder and Young Patients by Flow Cytometry

应用流式细胞仪分别对３３例青年人及５６例老年人大肠癌细胞ＤＮＡ含量进行了比较分析。结果表明，老年人大肠癌ＤＮＡ非二倍体癌发生率为４２．８％（２４／５６），青年人为７５．５％（２５／３３）．前者的非二倍体癌发生率显著地低于后者（Ｐ＜０．００１）。老年组大肠癌细胞ＰＩ均值为１４．８％，而青年人组为２０．９％，后组明显高于前组（Ｐ＜０．０１）。另外，老年人的管状腺癌及ＤｕｋｅｓＣ期癌二倍体发生率明显高于青年人（分别Ｐ＜０．０５）．除乳头状腺癌外，老年组各病理类型的细胞ＰＩ均值明显低于青年组．本组的结果提示，从分子生物学角度来看，青年人大肠癌是一种恶性度高及侵袭性强的肿瘤，而老年人大肠癌则反之．

Cellular DNA content of colorectal carcinoma from the elder and young patients wsa analyzed by flow cytometry(FCM).The results showed that the incidence of nondiploid tumor from the elder patients was 42. 8% (24/56) and from the young patients was 75. 5% (25/33). The incidence of nondiploid tumor in the young patients was significantly higher than that in the elder patients (P<0. 01).Mean value of cell proliferative index(PI) of colorectal carcinoma from the elder patients was 14.8% and from the young patients was 20. 9% ,the former was remarkably lower than the latter (P<0. 01).Compared with the young patients,the incidence of diploid in tubalar carcinoma and Dukes C stage from the elder patients was substantially higher(P<0. 05 , respectively), however, mean value of cell PI in all pathological factors but papillocarcinoma was notably lower (P<0. 05～0. 01).The present results suggested that the colorectal carcinoma in the young patients are more malignant and aggressive than that in the elder patients from the molecullar biological point of view.