外源性神经生长因子对神经在肌肉桥接物中再生的影响

NERVE REGENERATION IN THE INTERPOSED MUSCLE GRAFTS:THE INFLUENCE OF NERVE GROWTH FACTOR

自体冻融骨髂肌（ＦＴＭＧ）作为神经桥接体的实验是１９８６年Ｇｌａｓｂｙ首先报道，是基于此种非神经移植体的某些显见的优点，如轴突长入阻力小，有着与自体神经内膜管结构相似的肌基底膜管结构，可引导轴突向远端生长，但机械导向尚不能提供神经再生的全部必要条件。采用在冻融骨骼肌桥接物中加入外源性ＮＧＦ的方法，修复兔坐骨神经缺损，应用电生理学、组织学、免疫组织化学、图像分析、透射电镜等测试方法，发现早期神经轴突长入桥接体的速度及成熟程度明显优于单纯自体冻融骨骼肌桥（ＦＴＭＧ），为探索非神经组织桥接神经再生，及自体变性骨骼肌桥接物的临床应用，提供了基础实验依据。

Abstract Since 1986 free thawed muscle grafls(FTMG) had been used as nervous tissue grafts to brige the gap of the peripheral nerve. The exogenous NGF was applied in the FTMG to study the enhancement of for the nerve regeneration.A 2.0 cm segmen of the sciatic nerve was removed bilaterally in 24 male adult New Zealand rabbits. The gaps were bridged by FTMG. A dose of 30ng mouse NGF in Ringer's solutiop was injected into the grafts on one side and the Ringer's solution was injected into the other side as controls. The nerve conduction velocity histologica examination andimage pattern analysis of the axons by computer were investigated 4, 8,12, and 24 weeks following the surgery.The results show that the myotubes of the FTMG can guide the axongrowth in the early stage. NGF-injected side demonstrated a higher density distribu tion of axon growth and a rapid nerve regen eration rate, Preliminary report indicates that local application of NGF in FTMG may enhence the nerve regeneration.