摘要
ＴＮＦα系单核－巨噬细胞产生的细胞因子，具有明确促进纤维母细胞增殖的作用，但对胶原产生的影响文献报道不尽相同。本文研究并比较了重组ＴＮＦα对ＮＩＨ／３Ｔ３细胞及大鼠肝贮脂细胞增殖，Ⅰ、Ⅲ型前胶原基因表达的影响，结果发现ＴＮＦα具有促进上述两种细胞增殖作用，且具剂量依赖性。原位杂交显示，在单个细胞水平，ＴＮＦα可抑制或降低Ⅰ、Ⅲ型前胶原ｍＲＮＡ水平的表达。本研究提示肝贮脂细胞与纤维母细胞类似，接受ＴＮＦα调控，ＴＮＦα在肝纤维化始动激活及调控中具重要作用。
NFαwhich was produced mainly by
mononuclear cells and macrophages had definiteeffect on fibroblast proliferation,but its effect
on collagen production was controversial.Theeffect of recombinant TNFα on NIH/3T3 cell and
rat lipocytes proliferation as well as typeⅠand type Ⅲ procollagen gene expression were
studied.The results showed that TNFαstimulated the proliferation of NIH/3T3 cell and lipocyte
in dose-dependent and inhibited thetypeⅠand type Ⅲ procollagen expression at the level of
individual cell. The study revealedthat rat liver lipocyte is comparable with NIH/3T3 cell in the
presence of rTNFα. TNFα isone of the important mediators in the fibrosis initiation and
regulation.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1994年第1期25-27,共3页
Chinese Journal of Digestion
