摘要
采用３Ｈ－胸腺嘧啶掺入研究了血管活性肠肽（ＶＩＰ）对我院所建一株人胰腺癌细胞生长的影响，并对该细胞株进行ＶＩＰ受体、受体后细胞内介质ｃＡＭＰ和多胺产生的研究。发现ＶＩＰ在１０－８Ｍ和１０－７Ｍ对具有ＶＩＰ受体的人胰腺癌细胞有明显促生长作用，且成浓度依赖关系，这种作用可被其受体拮抗剂（４－Ｃｌ－Ｄ－ｐｈｅ６，Ｌｅｕ１７）ＶＩＰ所阻断；ＶＩＰ能显著促进本株人胰腺癌细胞细胞内ｃＡＭＰ及多胺的产生，表明ＶＩＰ的促生长作用可能与细胞内ｃＡＭＰ和多胺的生物合成增加有关；本株细胞在培养过程中可向培养基中分泌一定量的ＶＩＰ，ＶＩＰ抗体能抑制其在无血清培养基中生长，提示ＶＩＰ对本株人胰腺癌细胞的生长有自分泌调节作用。
In the present study,the effect of VIP on the
growth of a human pancreatic carcinomacell line (PU- PAN-1)was determined by means of
tritiated thymidine incorporation. VIPreceptors, intracellular cAMP and polyamines were
investigated.The results indicated thatVIP at concentrations of 1O-8M to -7Mcould significantly
stimulate the growth of PU- PAN- 1cells ; this effect was dose-dependent and could by
abolished be VIP receptor antagonist,4-Cl- D- Phe6 ,Leu17- VIP,suggesting the mediation of VIP
receptors.VIP could markedly elevate thelevel of intracellular cAMP and polyamines in PU-PAN-
1 cells,indicating the growth-prom- oting effect stimulated by VIP may be via rapid increase in
biosynthesis of cAMP andpolyamines. In addition, the VIP-antibody could inhibit the growth of
PU-pAN- 1 cells inserum- free culture medium.The above results suggest that this pancreatic
carcinoma cell linehas autocrine regulatory effect of VIP.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1994年第3期128-132,共5页
Chinese Journal of Digestion
基金
国家自然科学基金
关键词
血管活性肠肽
自分泌调节
胰腺肿瘤
Vasoactive intestinal
peptide
VIP receptors
Human pancreatic carcinoma cells
Polyamines
Autocrine regulation.
