胃癌单克隆抗体免疫交联物内化研究

INTERNALIZTION OF MONOCLONAL ANTIBODY IKMUNOCONJUGATES AGAINST GASTRIC CANCER

综合应用追踪技术对多种胃癌单克隆抗体免疫交联物在人胃癌培养细胞及临床胃癌实体瘤细胞中的内化进行了动态示踪定位，重点探讨了交联物中“弹头”的内化转运途径及命运，同时观察了环己哌啶对内化转运过程的影响。结果表明，内化是影响胃癌导向治疗效果的重要环节之一，是交联物与靶细胞作用的一种主要模式，内化研究将是克服胃癌导向治疗难关的主攻方向之一。

ith multiple tracing procedures,internalization of monoclonal antibody immunoconju-gates against gastric cancer was sequentially followed in cultures of human gastric cancerand in isolated tumor cells from gastric cancer specimen,with special emphasis on theintracellular trafficking and the fate of“warheads”in the immunoconjugates. Furthermore,the effects of perhexiline on the internalization processes were also observed.The resultsdemonstrate that internalization represents one of the major factors involved in the efficacyof immunotargeting therapy of gastric cancer and is a basic interaction mode betweenimmunoconjugates and target cells. We conclude that internalization will become a subject ofintensive investigation in exploring the maneouvres to overcome the obstacles encountered inthe targeting therapy of gastric cancer.