汉防己甲素对大鼠肝细胞、贮脂细胞DNA及胶原合成的影响

EFFECTS 0F TETRANDINE ON DNA AND C0LLAGEN SYNTHESIS OF RAT IT0 CELLS AND HEPATOCYTES

以３Ｈ－胸腺嘧啶（３Ｈ－ＴｄＲ）、３Ｈ－脯氨酸掺入量及掺入抑制率测定的方法，观察汉防己甲素对离体大鼠肝细胞、贮脂细胞ＤＮＡ及胶原合成的影响。结果表明，在１０～５０ｕｇ／ｍｌ浓度范围的汉防己甲素能明显抑制细胞内ＤＮＡ及胶原合成，抑制程度与浓度、作用时间均呈正相关。结果提示，汉防己甲素抗肝纤维化作用的机制之一是抑制肝内贮脂细胞、肝细胞增殖及合成胶原。

In order to investigate theanti-hepatofibrotic effects of tetrandrine,the authorsobs erved the effects of tetrandrine on the incorporation of 3H-TdR and 3H-proline into cultured rat Ito cells and hepatocytes.The results revealed that tetrahdrine in concentrations of l0￣50ug/ml can inhibit DNA synthesis and collagen production of the cells,and the inhibition is time-and dose-dependent。