汉防己甲素对肝纤维化大鼠线粒体的影响

EFFECT OF TETRANDRINE ON MITOCHONDRIA OF HEPATOFIBROSIS RATS

本文以四氯化碳建立大鼠肝纤维化模型，并用不同剂量的汉防己甲素进行早、晚期治疗，发现其小剂量能降低早、晚期治疗组模型线粒体的单胺氧化酶和Ｎ－乙酰β－Ｄ氨基葡萄糖苷酶的活性；增加线粒体对钙离子的主动摄取，减少其被动释放；增加线粒体内膜表层和中心处的流动性及巯基含量。由於大、中剂量效果不一，故提示小剂量汉防己甲素具有良好的线粒体保护作用和抗纤维化作用。

carbon tetrachloride was used to create the hepatofibrotic rat model.Different doses oftetrandrine(Tet)were used to treat these rats in the early and late hepatofibrotic stages. Weobserved that small doses of Tet could decrease mitochondrial (Mc)monoamine oxidase andN-acetyl-β-D-glucosaminidase activities in the early and late treatment groups;it couldincrease Ca2++ uptake and decrease Ca2+ efflux of Mc and meanwhile increase the fluidity ofsuperficial and paracentral parts of Mc inner membrane and its thiol contents. Large andmedian doses had variable effects. This experiment suggests that small dose of Tet have thebest protective effect on Mc as well as anti-hepatofibrotic effect.