骨形成蛋白与肌瓣修复骨缺损的研究

Repair of the Bone Defects of Tibia With Rotated Muscle Flap Reacted With Bovine Bone Morphogenetic Protein in Sheep

采用羊胫骨缺损作为模型，分别植入肌瓣＋骨形成蛋白和肌瓣。植入后第１，２，３，４周分批将羊处死，并进行Ｘ线摄片和病理组织学检查。结果表明：植入肌瓣＋骨形成蛋白组术后第２周即在缺损边缘及中央出现大量新骨，于第４周基本由新骨充填；而单纯肌瓣植入组由缺损边缘少量新骨生长，术后第４周缺损由新骨部分充填。说明植入骨形成蛋白＋肌瓣能够较快地修复骨缺损。

The experiment was designed to simulate the clinical bone defects-All experiments were performed on twelve immature sheep.Biolateral bone defects were created in the upperdiaphyseal shaft of tibia.One lateral defect served as the experiment,the other as the control.The musclitibilis anterior rotational flap and 50m8 of bBMP/iNCP consisted of No.5 ultra gelatin capsule were implanted into the experimental defects,the rotational muscle flap was implanted into the control one.Observations showed that the new bone was identified along the edge of the defects, as well as the central island of new formed bone in two-week experimental defect after operation.Four weeks after sursery repair were complete in experimental bone defects.In controls,two weeks after operation,new bone was only identified along the edges of the defects and the quantity of new bone was lower than those in experiments.