咪唑安定或丙泊酚拮抗戊四氮对突触传递的影响

MIDAZOLAM OR PROPOFOL ANTAGONIZING THE EFFECTS OF PENTYLENETETRAZOLE ON SYNAPTIC TRANSMISSION IN HIPPOCAMPAL CA1 NEURONS OF RATS

目的:观察咪唑安定或丙泊酚复合戊四氮对突触传递的影响。方法:分离大鼠海马半脑,切出400μm厚度的海马脑片,全细胞膜片钳记录戊四氮+咪唑安定组,戊四氮+脂肪乳剂组,戊四氮+丙泊酚组海马CA1区神经元兴奋性突触后电流(EP-SC)变化。结果:各组加入10mmol/L戊四氮均使EPSC降至基线值的35.0%左右;10μmol/L咪唑安定使EPSC幅值上升至基线值的86.2%,脂肪乳剂不改变EPSC,100μmol/L丙泊酚使EPSC值上升至基线值的71.7%。结论:戊四氮对正常突触传递具有抑制作用,咪唑安定或丙泊酚拮抗戊四氮抑制突触传递的作用,使已减小的EPSC有所升高。

Objective:To investigate the effects of midazolam or propofol on the whole-cell excitatory postsynaptic current of PTZ-incubated hippocampal slices. Methods:Wistar rats were killed by cervical dislocation and hippocampal slices(400μm) were prepared. EPSCs were recorded by whole-cell patch-clamp technique. After the base value of EPSCs was recorded for 10 min, 10 mmol/L PTZ was administered in perfusion for another 30 min.Moreover, 10 μmol/L midazolam or 90 μl intralipid or 100 μmol/L propofol was applied individually for 40 min.Result:PTZ significantly decreased the amplitude of EPSC. Midazolam reversed the effect of PTZ and increased the amplitude of EPSC to 86.2% of baseline. Intralipid didn't change the amplitude of EPSC,but 100μmol/L propofol increased the amplitude of EPSC to 71.7% of baseline.Conclusion:PTZ destroys the normal synaptic transmission and reduces the amplitude of EPSC. Midazolam or propofol can antagonize the effects of PTZ and reverse EPSC.