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RNAi-基因敲除技术在妇科领域中的应用 被引量：1

RNAi-gene knock-down technique and its application in gynecology
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摘要 RNAi是存在于真核生物体内的一种阻断外源基因表达的自我防御体系。它是由双链RNA介导的使与其互补的具有同源序列的单链RNA进行特异性的降解。随着这几年RNAi研究的不断深入,RNAi的干涉机制逐渐被阐明。该文介绍了RNAi的干涉机制和在妇科肿瘤领域中的应用进展。RNAi技术必将成为妇科肿瘤领域基因功能研究、细胞信号传导以及基因治疗的一种强大的新型工具。 RNAi represents an evolutionarily conserved cellular defense mechanism to control the expression of foreign genes in most eukaryotes. RNAi is triggered by double-stranded RNA(dsRNA) and causes sequence-specific mRNA degradation of homologous single-stranded target RNAs in response to dsRNA. With further studies on RNAi in recent few years, more intervention mechanisms have been elucidated gradually. This paper reviewed advancement in study on intervention mechanism of RNAi technique and its application in gynecology. It provides a new powerful tool for studies on gene function, cell signal conduction and gene therapy in gynecological field.[
作者 鲁艳明 张淑兰
机构地区 中国医科大学附属第二临床学院
出处 《国外医学（妇幼保健分册）》 2005年第3期186-189,共4页 Foreign Medical Sciences (Section of Maternal and Child Health)
基金 辽宁省社会发展项目基金资助项目(2001225002)
关键词 RNAI 宫颈癌 卵巢癌 基因治疗 RNAi siRNA cervical carcinoma ovarian carcinoma gene therapy
分类号 R737.3 [医药卫生—肿瘤] R711 [医药卫生—妇产科学]
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参考文献15

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同被引文献23

  • 1刘杰凤,王志辉,李春晖.RNA干扰用于基因治疗的研究进展[J].生物技术通讯,2006,17(2):276-278. 被引量：4
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  • 10Wilson JA,Jayasena S,Khvorova A,el al.RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells[J].Proc Natl Acad Sci USA,2003,100:2783-2788.

引证文献1

国外医学（妇幼保健分册）
2005年 第3期

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