贵州省区试小麦品系Glu-1位点的PCR研究

Study by PCR Technology on Glu-1 Locus in Regional Testing Wheat Lines of Guizhou Province

本文利用SDSPAGE电泳方法鉴定了2001～2003年参加省区试的18份小麦品系的高分子量麦谷蛋白亚基组成,并根据GluDly位点的基因序列设计的一对引物,通过PCR技术对这些小麦品系的基因组DNA进行扩增,可以准确鉴别亚基组成为2+12和5+10的不同小麦品系。同时,用不同亚基组成亲本的杂交后代进行扩增,可以从F2后代群体中选择含有5+10亚基的单株,为分子标记辅助选择在小麦品质育种中的应用提供了基础。

The composition of high molecular weight glutenin subunit (HMW-GS) of eighteen regional testing wheat lines in Guizhou province during 2001-2003 were identified with the method of SDS-PAGE electropherogram. With a pair of synthetic primers by specific variation for Glu-Dly, different wheat lines with subunit pair 2+12, 5+10 can be distinguished clearly by genome DNA being amplified with PCR approach. At the same time, amplifying the hybrids whose parents contained different subunits, the plants contained subunit pair 5+10 can be selected from F 2 generation .PCR approach provided a base on molecular marker-assisted selection in early generation of wheat breeding program.