基于RP的骨组织工程支架构造及生物学特性分析

Fabrication of Bone Tissue Engineering Scaffolds Based on RP and Analysis of Biological Properties

应用三维CAD软件设计支架和相应的模具结构,通过光固化快速成形技术制造出树脂模具,并在模具中填充磷酸钙(CPC)生物材料,然后通过热分解方法去掉树脂模具,得到具有可控微管道结构的骨组织工程支架。该方法克服了传统构造方法中支架内部微管道结构不可控的缺点,为制造出更有利于细胞/组织长入和成活的支架三维空间结构提供了一个更理想的方案。INSTRONMicrotester试验设备上测得支架的最大抗压强度为7.12MPa;测得其表面粗糙度Ra=2.16μm。扫描电镜观察支架表面微结构特征,能谱分析测出支架中所含钙元素和磷元素的摩尔比是1.59∶1。结果表明所构造的支架具有良好的生物学特性。

Negative molds based on the scaffold designs were fabricated using stereolithography RP technique. Calcium phosphate cement (CPC) slurry was cast into the resin molds. After pyrolysis, the bone tissue engineering scaffolds with controlled internal pore architectures were obtained. This method can overcome the defects that internal microchannel structure of scaffolds fabricated by traditional methods are uncontrollable, so it provides an ideal strategy to fabricate a suitable 3D structure of scaffolds beneficial to cell/tissue in growth and survival. The scaffolds were tested on an Instron Microtester and the maximum compressive strength measured is 7.12MPa. The surface roughness Ra value measured is 2.16. Furthermore, the surface microstructure characteristics of the scaffolds were also observed under scanning electron microscope (SEM). The mol ratio of Ca and P measured by energy spectrum analysis is 1.59:1. The results proves that scaffolds have favorable biological properties.