红树林沉积物中的微生物对苯并[a]芘的降解研究

Biodegradation of Benzo[a]pyrene by Mangrove Microbial Consortium

采用高效液相色谱(HPLC)结合紫外检测研究了一组筛选自红树林沉积物中的混合菌,该混合菌能以苯并[a]芘(BaP)作为唯一碳源和能源进行代谢,结果表明,当BaP浓度为20mg·L-1时,培养63d后有32.85%的BaP被降解.经分离,此混合菌中包含5种菌,16SrDNA序列测定结果与GenBank数据库进行同源性比较,显示M151可能为一新种,其余4株菌分别为红球菌属(Rhodococcussp.),微小杆菌属(Exiguobacteriumsp.),节杆菌属(Arthrobactersp.),芽孢杆菌属(Bacillussp.),绘制了系统发育树,并对其系统发育进行分析.同时还强调了红树林微生物在BaP污染环境生物修复中作用的重要性.

A microbial consortium which can utilize benzo[a]pyrene(BaP) as the sole source of carbon and energy was isolated from mangrove sediment and enriched in liquid medium.High-performance liquid chromatography (HPLC) with UV detection detected that 32.84% of BaP was degraded by the consortium after 63 days′ incubation in BaP-MSM medium. Five strains of the consortium were isolated with spray-plate method.All these five 16S rRNA genes were PCR amplified,sequenced and submitted to the GenBank database,searching for the similarity with sequences of other bacteria.Results indicate that M1 5-1 was probably a new genus and the other four are Rhodococcus sp.,Exiguobacterium sp.,Arthrobacter sp.and Bacillus sp.Phylogenetic tree was conducted,based on the five 16S rDNA sequences and their neighbors in the database.The importance and efficiency of mangrove microorganisms in the bioremediation of BaP polluted environment were also discussed and emphasized in the paper.