鼠肺门剥脱后肺淋巴引流中断对抗体反应的影响

The Influence of Interrupted Pulmonary Lymph Drainage on Antibody Responses in Hilar Stripped Rats

肺移植后肺感染的发生率比其它器官移植后明显增高，这提示移植肺的免疫反应可能受损。为了研究肺淋巴引流中断对抗体反应的影响，我们用肺门剥脱鼠作为模型，设计了３个实验：（１）用着色淋巴造影术目视检查肺门淋巴管的再生；（２）在支气管内滴注碳粒子后观察粒子从肺到肺引流淋巴结的运输；（３）用羊红细胞使肺免疫后测定抗体反应。结果显示肺门淋巴管被肺门剥脱术所中断，在术后７天开始再生；在术后第１周粒子运输到肺引流淋巴结受阻，此后逐渐恢复正常；在肺门剥脱后７天和１０天免疫的鼠血清抗体反应缺如或低下，其后１月内恢复。由此得出结论，只要抗原不能经淋巴管从肺运送到肺引流淋巴结，肺门剥脱肺内的抗原抗体反应就受到损害。该发现可部分地解释为什么在肺移植后最初几周内经常发生肺感染。

The incidence of pulmonary infection after lung transplantation is significantly higher than after other organ transplantations. This suggests that the immune response in transplanted lungs is impaired.To investigate the influence of lymphatic interruption on antibody responses, we designed three experiments using hilar stripped rats as a model: 1.visual detection of regenerated hilar lymphatics by chromolymphography, 2. observation of transport of particles from the Jung to the lung-associated lymph nodes after intrabronchial instillation of carbon particles, 3. assessment of antibody responses after lung immunization with sheep red blood cells.The results showed that hilar lymphatics were interrupted by hilar stripping and began to regenerate from day 7 after operation. Transport of particles to the lurg-associated Jymph nodes was blocked during the first week after operation but gradually returned to normal levels thereafter. Serum antibody titers were absent or low in the rats immunized on days 7 and 10 after hilar stripping, subsequently antibody responscs gradually recovered in one month.We concluded that antibody responses to antigens in hilar stripped lungs were impaired as long as the antigens could not be transported through lymphatics from the lung to the lung-associated lymph nodes. These findings can explain in part why pulmonary infections occur so frequent in the initial weeks after lung transplantation.