大蒜种质资源遗传多样性的分子标记研究

Assessment of Genetic Diversity of Garlic(Allium sativum L.) by the Technique of Molecular Marker

本研究利用RAPD和ISSR两种分子标记技术,对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究.从30个RAPD引物当中,筛选出11个具有多态性的引物,共扩增出多态性带224条,多态性条带比率为41.18%,从12个ISSR引物中筛选出5个具多态性的ISSR引物,共扩增出多态性带121条,多态性条带比率为50.21%.对RAPD和ISSR两种标记分别运用Nei指数法,计算出10个大蒜品种的平均遗传距离为0.28和0.32.根据这两种标记的结果,采用UPGMA进行聚类分析,得到与生物学分类地位基本一致的结果.结果表明,在实验稳定性上,ISSR优于RAPD,且ISSR比RAPD能检测到更多的遗传变异,RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究.

Random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat (ISSR) methods were used to detect genetic variation of 10 varieties of garlic (Allium sativum L.)from different regions and provinces of China.11 RAPD primers generated 224 polymorphic bands.Percentage of polymorphic bands (PPB) of RAPD was 41.18%.5 ISSR primers generated 121 polymorphic bands.PPB of ISSR was 50.21%.The result of UPGMA cluster analysis based on two molecular markers data was similar to the result based on the biologic characteristic.Comparing with the two marker systems it showed that ISSR was better than RAPD in terms of reproducibility and ability of detecting genetic polymorphism.Consequently,the results showed that RAPD and ISSR could be applied to detect genetic diversity among garlic.