0#柴油水溶性成分对僧帽牡蛎(Ostrea cucullata)谷胱甘肽硫转移酶活性的影响

Effect of Water-soluble Fraction of No.0 Oil on Glutathione S-transferase in Ostrea cucullata

在实验条件下,将僧帽牡蛎(Ostreacucullata)分别置于含有低(0.5mg/L)、中(2mg/L)和高(5mg/L)3种浓度0#柴油水溶性成分(watersolublefraction,WSF)的海水中,在污染后1,4,7,11,15d采样,15d后转入清洁海水中进行6d的恢复实验,采样.测定消化腺和鳃谷胱甘肽硫转移酶(GST)的活性.结果表明:正常生理条件下鳃GST活性高于消化腺;消化腺和鳃GST活性随污染时间的延长先被诱导后逐渐下降,并存在一定的剂量效应关系,可作为监测海洋石油污染的生物标志物;解除污染后,GST活性恢复到对照组水平.

In experimented condition,oyster Ostrea cucullata was exposed to different concentrations of water-soluble fraction (WSF) of NO.0 diesel oil (0.5,2,5 mg/L) for 15 d,and then transferred into clean sea water for 6d restoring experiment.The glutathione S-transferase (GST) activities in the digestive gland and the gill of 1,4,7,11,15,21 d sample were measured.The results show:In control group,the gill GST is higher than digestive gland;With the prolonged exposure,the digestive gland and gill GST are induced first,and then reduce gradually.The dose-effect relationship occurres in 15 d sample;GST activity of all concentration groups are recovered to the level corresponding to that of the control group after recovery experiment.According to the data,the GST of marine bivalve could be a biomarker to monitor marine oil pollution.