肺鳞癌DNA含量定量分析及其临床意义

A Quantitative Study on DNA Content of Squamous Cell Lung Cancer by Image Analysis Technique

应用图象分析技术对４２例肺鳞癌细胞ＤＮＡ含量原位定量测定。结果显示，肺鳞癌ＤＮＡ干系处于二倍体／近二倍体（Ｄ／ＮＤ）者８例，占１９％；ＤＮＡ干系处于异倍体者（ＡＮ）３４例，占８１％。瘤体直径＞３ｃｍ、淋巴结癌转移者，其平均ＤＮＡ含量、异倍体检出率皆明显高于瘤体直径≤３ｃｍ和淋巴结阴性者。平均ＤＮＡ含量、异倍体检出率随肿瘤组织学分级的增高而升高。Ｄ／ＮＤ型肺癌５年生存率明显高于ＡＮ型肺鳞癌。结果说明，肺鳞癌ＤＮＡ含量测定有助于了解其恶性程度和发展趋势，可为临床诊断、治疗和估计预后提供依据。

DNA content was quantitatively determined in 42 resected specimens of squamons cell lung cancer by image analysis technique using paraffin-embedded sections stained with Feulgen method.The results showed that diploid/near diploid DNA content (D/ND)and aneuploid DNA(AN)were found in 19% and 81% of the specimens of squamous cell lung cancer respeetively.The percentages of aneuploid DNA and mean DAN content of these tumors in which diameter was>3cm,lymphaden metastases were higher than those≤3cm with no lymphaden metastases. A correlation was found between both the mean DNA content and percentage of aneuploid DNA and pathologic grading of lung cancer.It is suggested that the quantitative analysis of DNA content in the squamous cell lung cancer may be helpful in evaluating its grade of malignancy and prognosis. DNA content analysis also showed that patients with diploid tancers had a better prognosis and longer survital than those with distinct aneuploid.