用流式细胞仪测定小儿急性白血病细胞DNA及其临床意义研究

CLINICAL SIGNIFICANCE OF THE FLOW CYTOMETRY INTHE ESTIMATION OF DNA IN THE CHILDREN ACUTELEUKEMIA CELL AND DETECTION OF MINIMALRESDUAL DISEASE

应用流式细胞仪（ＦＣＭ）对９４例急性白血病（ＡＬ）骨髓单个核细胞（ＭＮＣ）进行了ＤＮＡ含量测定。在４７例初诊／复发ＡＬ中，５０％（１７／３４）的急性淋巴细胞白血病（ＡＬＬ）及３８．５％（５／１３）的急性非淋巴细胞白血病（ＡＶＬＬ）检出ＤＮＡ异倍体；４７例ＡＬ完全缓解（ＣＲ）期患儿异倍体检出率为１０．６％。１４例非白血病患儿无异倍体检出。ＡＬ初诊病例的Ｃ＿０／Ｇ＿１％高于ＣＲ及对照组，Ｓ％及（Ｓ＋Ｇ＿２）／Ｍ％低于ＣＲ及对照组；复发病例（Ｓ＋Ｇ＿２）／Ｍ％高于初诊组；在ＡＬ－ＣＲ组，Ｓ％和（Ｓ＋Ｇ＿２）／Ｍ％均与对照组无显著性差异。２０例ＡＬ的自身对照发现，１１例初诊、复发时的异倍体在ＣＲ期消失。

DNA content estimation has been carried out in themononuclear cell of the bone marrow in 94 cases of acuteleukemia (AL) with flow cytometry. In 47 cases of ini-tial/relapse acute leukernia , DNA aneuploid had been es-timated out in 50% (17/34) of acute lymphocyticleukemia (ALL) and 38. 5% (5/13) of acute nonlym-phocytic leukemia (ANLL) ; aneuploid estimation rate in47 cases of AL in CR was 10. 6%. No aneuploid was es-timated out in 14 cases other than leukemia. G_0/G_1% inAL initial cases was higher than that in CR and control;S% and S+G_2/M/% were lower than that in CR andcontrol; S+G_2/M/% in relapse cases was hiunher thanthat in initial cases; S% and S+G_2/M/% in AL-CRgroup were of no significant difference than that in con-trol. Self-control in 20 cases of AL revealed that ofwhorn the 11 inrtial cases the aneuploid in relapse wasdisappeared while in CR.