聚合酶链反应检测再生障碍性贫血血清中乙型和丙型肝炎病毒

DETECTION OF SERUM HEPATITIS B VIRUS(HBV) ANDHEPATITIS C VIRUS (HCV) IN APLASTIC ANEMIA BYPOLYMERASE CHAIN REACTION

应用聚合酶链反应（ＰＣＲ）检测了３７例非肝炎相关再生障碍性贫血（ＮＨＡＡ）和１８例肝炎相关再生障碍性贫血（ＨＡＡ）血清中的乙型肝炎病毒ＤＮＡ（ＨＢＶＤＮＡ）、丙型肝炎病毒ＲＮＡ（ＨＣＶＲＮＡ）。结果显示：ＮＨＡＡ的ＨＢＶＤＮＡ检出率为１３．５％（５／３７），ＨＡＡ为２２．２％（４／１８），两者比较Ｐ＞０．０５。ＮＨＡＡ的ＨＣＶＲＮＡ检出率为２．７％（１／３７），ＨＡＡ为３８．９％（７／１８），两者比较Ｐ＜０．０１。丙型肝炎相关再生障碍性贫血的预后较差。提示：ＨＣＶ感染与ＨＡＡ关系密切。

erum HBV DNA and HCV RNA were determined in37 cases of non-hepatitis-associated aplastic anemia(NHAA) and 18 cases of hepatitis-associated aplasticanemia (HAA) by polymerase. chain reaction (PCR ).The relationship of hepatitis virus to the prognostic sig-nificance of HAA was investigated. The results showedthat: HBV DNA was found in 13. 51 % (5/37) NHAA ,and in 22. 22%(4/18) HAA, (P>0. 05). HCV RNAwas found in 2. 7% (1/37) NHAA, and in 38. 89% (7/19) HAA, (P<0. 01 ). HAA with HCV-positive tend-ed to have poor prognosis. The finding suggested thatinfection of HCV was related to the occurrence of HAA.