响应面设计法优化腺苷发酵培养基

Optimization of Medium Components for Adenosine Production by Bacillus subtilis Using Response Surface Design

利用响应面方法对枯草芽孢杆菌生产腺苷的培养基进行了优化。首先用两水平因子实验对培养基组分酵母粉、玉米浆、KH2PO4、MgSO4、(NH4)2SO4对产苷的影响进行评价,结果表明:主要影响因子为酵母粉、KH2PO4和(NH4)2SO4。根据实验结果对主要影响因子的大致范围进行估计,选定合适的浓度作进一步试验,然后用中心组合设计及响应面分析确定主要影响因子的最佳浓度。通过实验发现当酵母粉15g/L,KH2PO46g/L,(NH4)2SO410g/L时,腺苷产量达到7.42g/L,比优化前的5.77g/L提高了28.6%。

Response surface design was used to optimize the medium components for adenosine production by Bacillus subtils. Firstly, effects of yeast extract, corn steep liquor, KH2PO4, MgSO4, (NH4)2SO4 on adenosine production were evaluated using a 2-level factorial design. Among the components investigated, yeast extract, KH2PO4 and (NH4)2SO4 played important roles in adenosine production, while others had little effect. Then a central composite design was used to optimize the significant factors and the results were shown in response surface plots. When the medium comprised (g/L) yeast extract 15, KH2PO4 6 and (NH4)2SO4 10, the adenosine yield was 7.42 g/L, which was 28.6% higher than the control.