CIK细胞的制作及对不同肿瘤细胞株体外抗肿瘤作用的研究

Preparation and cytotoxic effects of cytokine-induced killer cells on different tumor cell lines in vitro

目的:制备细胞因子激活杀伤(CIK)细胞并观察其生物学特性及对不同肿瘤细胞株的杀伤作用。方法:外周血单个核细胞用无血清培养基经干扰素γ(IFNγ),CD3McAb,白介素2(IL2),白介素1α(IL1α)诱导,制作CIK细胞,以淋巴因子激活的杀伤细胞(LAK)作为对照,流式细胞仪检测CIK细胞的免疫表型,MTT法检测其对不同肿瘤细胞株的杀瘤活性。结果:CIK细胞与LAK细胞相比增殖速度快、最终增殖倍数高。CIK细胞主要由CD3和CD56双阳性细胞构成。CIK细胞对多种肿瘤细胞具有较强的杀伤活性。结论:CIK细胞过继免疫治疗是一种非常有前景的抗肿瘤治疗方法。

Objective: To prepare cytokine-induced killer (CIK) cells and observe its biological features and cytotoxic effects on different tumor cell lines in vitro. Methods:CIK cells were induced by culturing peripheal blood mononucleor cells in the AIM-V medium containing some cytokines such as interferon-γ(IFN-γ), McAb against CD3, interleukin-2(IL-2) and interleukin-1α(IL-1α). Taking lymphokine activated killer(LAK)cells as control. Phenotypic characterization. CIK cells were analyzed by using flow cytometric analyser. The cytotoxicity of CIK cells against different tumor cell lines was determined by MTT assay. Results:CIK cells increased significantly compared with LAK cells. The CD3 and CD56+ T cells were the major conponeuts of CIK cells. CIK cells possessed higher antitumor cytotoxic activity against mang tumor cell lines. Conclusion:CIK cells may be the best method in adoptive antitumor immunotherapy.