重组人转化生长因子－β_1对人白血病细胞系（J6－1，J6－2，Namalva）增殖的调节

REGULATION OF rH-TGF-β_1 ON PROLIFERATION OF HUMAN LEUKEMIC CELL LINES J6-1,J6-2 ANDNAMALVA

用ＤＮＡ－ｍＲＮＡ原位杂交和常规细胞生物学方法研究重组人转化生长因子－β＿１（ｒｈ－ＴＧＦ－β＿１）对Ｊ６－１，Ｊ６－２和Ｎａｍａｌｖａ细胞的作用。ｒｈ－ＴＧＦ－β＿１对Ｊ６－１细胞增殖抑制作用呈明显的剂量依赖和双相时间依赖关系。对Ｊ６－１细胞的抑制主要通过抑制ＤＮＡ合成，对Ｊ６－２的抑制则通过抑制ＤＮＡ合成和促进凋落，Ｎａｍａｌｖａ细胞对它不敏感。ｒｈ－ＴＧＦβ＿１对细胞基因表达的影响也因株而异：同时下调三个细胞系ｃ－ｍｙｃ、ＬＩＦ和ｐ５３的表达，上调ｖ－ｓｉｓ的表达；对Ｊ６－２和Ｎａｍａｌｖａ的众多基因表达以下调为主，却上调Ｊ６－１细胞多个癌基因的表达，提示ＴＧＦ－β可能使某些白血病细胞更趋恶性。

NA-mRNA in situ hybridization was used to demon-strate the effects of rh-TGF-β_1 on human leukemic celllines J6-1,J6-2 and Namalva. TGF-β_1 inhibited J6-1 cellproliferation in a dose-dependent and bi-directional time-dependent manner. Inhibition of cell division was foundin J6-1 cells , while both inhibition of division and pro-motion of apoptosis were observed in J6-2 cells whentreated with rh-TGF-β_1.No Significant change wasfound in Narnalva cells. The effects of TGF- β_1 on geneexpression varied among the cell lines.C-myc,LIF andp53 genes were down regulated while v-sis gene was up-regulated in all the 3 cell lines. TGF- β_1 down- regulatedmost gene expression in J6-2 and Namalva celis,but up-regulated most of them in J6-1 cells. It suggested thatTGF-β_1 might turn some leukemic cells more malignant.