成年小鼠的耐受机制及其在异基因骨髓移植中的应用

MECHANISM OF TOLERANCE INDUCED IN ADULT MICEAND ITS APPLICATION TO ALLOGENEIC BONEMARROW TRANSPLANTATION

静脉注射异基因脾细胞和骨髓细胞加腹腔注射环磷酰胺和抗胸腺细胞血清可诱导成年小鼠异基因皮肤移植耐受。体内、外细胞转移实验均未显示耐受小鼠脾细胞中存在抑制细胞活性。直接荧光染色的流式细胞仪分析结果表明，耐受小鼠胸腺内形成了微量混合嵌合体。以耐受小鼠作为异基因骨髓移植的供体，可完全避免移植物抗宿主病的发生，耐受骨髓可在受体中建立稳定的嵌合体，使受体小鼠对供体来源的肿瘤产生了特异耐受。

sing intravenous injection of allogeneic spleen cellsplus bone marrow cells followed by intraperitoneal injec-tion of cyclophosphamide and antithymocyte serum,weachieved a long term survival of skin allograft in adultmice. Invivo and in vitro transfer experiments did notshow any suppressive activity in the spleen cells of thetolerant mice, FACS analysis of direct immunofluo-rescence test sbowed that a mixed microcbimerismdevel-oped in the thymus of the tolerant mice,which suggest-ed that the mechanism of the tolerance might be mainlyinvlved in the.clonal deletion,When tolerant mice wereued as donors in the allogeneic bone marrow transplan-tation, the graft versus host disease(GVHD)could becompletely avoided in the recipients. The reconstitute re-cipients established a stable chimerism and could toleratethe growth of donor-derived tumor graft.