脐血CD_(34)^+CD_(19)~－细胞与体外培养集落的相关性分析

CORRELATIVE ANALYSIS OF CD_(34)-POSITIVE CELLS OFCORD BLOOD WITH CFU-GM , BFU-E AND CFU-MIX

用两种单克隆抗体（单抗）标记脐血造血祖细胞表面抗原（ＨＰＣＡ，ＣＤ＿（３４））用流式细胞仪分析，并比较两种单抗标记的细胞与体外培养的粒单细胞集落形成单位（ＣＦＵ－ＧＭ），红系爆式集落形成单位（ＢＦＵ－Ｅ）和混合集落形成单位的相关性，结果表明脐血有核细胞中，抗ＨＰＣＡ－２－ＦＩＴＣ阳性的细胞占１．０５１０．７２％，Ｔｕｋ３（纯抗体）阳性细胞占２．０６±１．２５％，差别显著（Ｐ＜０．０５），每μｌ脐血两种单抗标记的细胞分别为９６．５６±５６．６４和２３１．４０±１６３．９３（Ｐ＜０．０５），变异系数依次为５８．４７％和６８．４３％。尽管抗ＨＰＣＡ－２－ＦＩＴＣ阳性细胞与阳性细胞数量呈显著正相关，但前者与ＣＦＵ－ＧＭ，ＢＦＵ－Ｅ，ＣＦＵ－Ｍｉｘ以及ＣＦＵｓ（ＣＦＵ－ＧＭ＋ＢＦＵ－Ｅ＋ＣＦＵ－Ｍｉｘ）均呈正相关，而后者仅与ＣＦＵ－ＧＭ，ＣＦＵｓ呈正相关。研究结果提示在检测造血祖细胞时，用抗ＨＰＣＡ－２－ＦＩＴＣ代替可降低假阳性，获得较好的细胞与ＣＦＵ间的线性关系。

wo anti-CD_(34)monoclonal antibodies (antiHPCA-2 ,Tuk_3) were used to label cord blood celis. The labellingcells were assayed by flow cytometry, and the relation-ship between the CD_(34) positive cells and the CFU-GM,BFU-E and CFU-Mix was analysed. The resultsshowed that the CD_(34) positive cells of cord nucleated cellslabeled by anti-HPCA-2 and Tuk, were 1. 05 ±0. 72%and 2. 06±1. 25 % (p<0. 05) , and the absolute numharsof CD_(34) positive cells per ul in cord blood were 96. 56±56. 64 and 231. 40 ±163. 93 (P<0. 05), respectively.Although the number of anti-HPCA-2 positive cells cor-related directly with that of Tuk_3 positive cells , the for-mer correlated with the number of CFU-GM , BFU-E ,CFU-Mix and the sum total of all the CFUs, while thelatter correlated only with the numbers of CFU-GM andBFUE. This study suggested that using anti-HPCA-2to detect the HPCA of cord blood cells instead of Tuk_3might decrease the false-positivity and obtain better lin-ear relationship of CD_(34) positive cells with colony formingunlts.