摘要
应用醛化的鸡红细胞经吸附释放方法获得纯化的新城疫病毒(NDV),经tritonX100处理并反复冻融制备新城疫ELISA抗原。应用抗鸡Ig轻链单抗1B7辣根过氧化物酶结合物建立了定量检测NDV抗体水平的ELISA方法。确立了将鸡血清100倍稀释检测ELISA效价(ET)的回归方程y=0.94128x+0.20677,r=0.98163,可用于定量测定。血凝抑制(HI)方法与ELISA方法的比较试验表明,ELISA法的敏感性是HI方法的3倍以上。应用建立的ELISA方法比较了卵黄、气管及胆汁中局部抗体与血清抗体的差异。
The indirect ELISA method for detecting antibody level to newcastle disease virus was developed by preparing coating antigen by absorbing and releasing virus from chicken blood cells, and enzyme conjugate of mouse against light chain of chicken Ig. A linear regression equation for detecting titer was developed under 100 fold dilution sera sample that was y=0.941 28 x+0.206 77,r=0.981 63. The ELISA titer was 3 times higher than that of hemagglutination inhibitor test (HI).Using ELISA method, the NDV antibody titre in sera was compared with that in yolk, bile, and tracheal, respectively.
出处
《中国兽药杂志》
2005年第6期22-26,共5页
Chinese Journal of Veterinary Drug
基金
天津市自然科学基金资助项目(013614911)
天津大学青年教师基金资助项目(5110110)
关键词
鸡新城疫
抗体
ELISA
chicken
newcastle disease virus
ELISA
