小麦中雄性不育同源序列的分离、鉴定及表达分析

Isolation,Characterization and Expression Analysis of Male Sterility Gene Homology Sequence in Wheat

利用拟南芥中已克隆的雄性核不育基因MS2和水稻中假定雄性不育蛋白的保守区域,设计一对简并引物,并在太谷核不育小麦可育株及不育株花药中进行扩增,得到了一条134bp的片段。以该片段为基础,通过电子延伸得到一个长为1 604bp的序列,该序列编码的氨基酸包含一段由200个氨基酸组成的雄性不育保守区。RT PCR结果表明,该雄性不育同源序列只在小麦可育花药中表达,而在小麦败育花药、叶片和根中不表达,说明该雄性不育同源序列为花药发育特异基因。

One 134 bp fragment was amplified in anthers of male sterile and fertile wheat using one pair of degenerated primer designed based on the conserved domain of MS2 gene in Arabidopsis thaliana and Oryza sativa ,and one 1 604 bp male sterility gene homology sequence was extended by in silico cloning based on the 134 bp fragment.The amino acids encoded by the male sterility gene homology sequence include a 200 amino acid conserved domain of male sterility,and this sequence expressed only in wheat male fertile anthers;no expression in male sterile anthers,roots and leaves.This research demonstrated that the cloned male sterile homology sequence is specific to wheat anther development.