70个水稻微卫星标记染色体位置的更正

Correction of 70 SSR Markers of Rice

微卫星标记(SSR)因其操作简单和稳定可靠的特点而成为一种重要的分子标记,被广泛应用于遗传作图和种质鉴定等方面。但其在染色体上位置的正确性将直接影响到基因定位的正确性和后续研究的方向。利用美国国家生物信息技术中心(NCBI)网站的Blast程序,将2740个SSR 标记的前后引物序列与水稻粳稻品种日本晴基因组进行比对,共发现70个标记位于另一条染色体,对这70个标记重新锚定的染色体进行了更正。这将有助于今后水稻分子标记遗传连锁图的正确构建。

Dense coverage of the rice genome with microsatellite markers was an invaluable tool for marker assisted breeding(MAS), positional cloning, and a wide range of evolutionary studies. Until 2002, a total of 2740 microsatellite markers has been mapped in rice molecular genetic map, approximately one microsatellite marker every 157kb. The accurate position of microsatellite markers in rice genome was a key factor for gene positional cloning, dissection for quantitative trait loci (QTLs) , and genetic analysis of correlated traits. When a genetic linkage map is constructed, the linkage relationship of some adjacent markers showed abnormal because they are located on different chromosomes. In order to identify on which chromosome all microsatellite markers are located, 2740 pairs of primer sequences specified for each marker were blasted to rice genome sequences (Oryza sativa ssp. japonica cv. Nipponbare).Using blastn program to search for short and nearly exact matches option, 70 markers were anchored on another chromosome according to the comparison of the chromosome position of hitted clone with chromosome position published. Because these markers represent an unique marker loci in genome, they have a relative important value for gene mapping. Their correct chromosomes were reported, and would contribute to gene positional cloning and construction of molecular markers genetic linkage map.