介绍两种鉴定白色念珠菌的方法

Two methods for identifying Candida albicans

应用含０．０１％阿尼林蓝ＷＳ染料的ＹＭ固体培基（ＹＭＡＢ）和以对－硝基苯酚－Ｎ－乙酰－β－Ｄ－氨基半乳糖苷（ＮＧＬ）为底物鉴定白色念珠菌（白念菌），同时进行芽管试验。生长于ＹＭＡＢ上的白念菌温育１２～１８小时后在伍德灯下可观察到黄绿色荧光。ＮＧＬ酶法温育９０分钟后，白念菌酶类可分解底物呈现明显黄色。两种方法均正确鉴定了５５例白念菌中的５４例（９８．２％）。阿尼林蓝法中，１株类星形念珠菌也出现荧光，特异性９２．８％，敏感性及预示值均高于芽管法。ＮＧＬ酶法中，７株近平滑念珠菌呈阳性，特异性５０％，但反应快速。将两种方法并用，可使正确鉴定率达１００％，且两种方法简便、价廉，易于推广。

M medium containing 0.01% Aniline Blue ws and PNP-N-Acetyl-β-D-galactoseminide wereused to identify Candida albicans. The result was compared with the germ tubing test. 54 from 5 5strains of C albicans were correctly identified by the 2 methods. With the Aniline Blue method,1 strainof C stellatoidea was also positive. In NGL method 7 strains of C parapsilosis were positive. When these 2 methods were used in combination,the correct identification rate reached 100 %.