摘要
建立了用特异性刺激原白细胞分化抗原３（ＣＤ＿３）单克隆抗体（单抗）诱导外周血淋巴细胞（ＰＢＬ）增殖转化、产生白细胞介素－２（ＩＬ－２）及ＩＬ－２受体（ＩＬ－２Ｒ）表达等检测Ｔ细胞功能的方法，并对其实验影响因素进行探讨。结果表明：ＣＤ＿３单抗诱导ＰＢＬ活化的过程与Ｔ细胞在体内的活化过程相符；ＣＤ＿３单抗的丝裂原作用所需浓度范围广、用量少；由ＣＤ＿３单抗诱导的ＰＢＬ转化能力受ＣＤ＿３单抗的浓度和培养时间的影响；ＣＤ＿３单抗与植物血凝素（ＰＨＡ）活化Ｔ细胞的效应，在Ｔ细胞增殖方面二者呈显著正相关，而在ＩＬ－２活性和ＩＬ－２Ｒ表达方面则无相关性。
his study extablished the methods to detect T cell's functions induced by a characteristic mitogen-anti-CD_3mAb, and their influential factors observed.T lymphocyte transfoimation,activity of IL-2and expression of IL-2R were determined, by means of the 3￣H-TDR incorporation, bioassay and immunoabsorption's method. The results show that the process of PBL activation that induced by anti-CD_3mAb tallied with T cell's in vivo: The range of concentration of anti-CD_3mAb induced PBL proliferation was wide and its dosage was little,PBL activation stimulated with anti-CD_3mAb was influ-enced by culture time and the concentration of anti-CD_3mAb.The results still show that G.I of anti-CD3mAb and PHA stimulating PBL proliferatlon was positlvely correlated,activity of IL-2and expression of IL-2R were not correlated.
