摘要
应用十二烷基硫酸钠-聚丙烯酰胺垂直板电泳及免疫印迹分析技术,以自制羊抗人载脂蛋白(a)[apo(a)]为第一抗体,辣根过氧化物酶标记兔抗羊IgG为第二抗体,建立了apo(a)多态性检测方法,并对方法学中某些因素进行了探讨。检测了190名健康人血清,按Utermann等的分型方法,检出5种apo(a)多态,共12种表型。其中B占2.1%,S_1占3.2%,S_2占18.4%,S_3占20.5%,S_4占30.5%。分子量范围在486000~741000之间,检出率为83.2%。
olymorphic forms of human apo(a)were determined by SDS-PAGE electrophoresis and immunoblotting.According to the nomenclature of Utermann et al, the authors found 12 different phenotypes ranging in molecular weight from 486 000 to 74 1 000 in the healthy subjects. Among them B-type was 2.1%, S_1-type 3.2%,S_2-type 18.4%,S_3-type 20.5%,S_4-type 30.5%,“null”16.8%. The de-tective rate was 83.2%.