摘要
应用胶体金探针建立了斑点免疫金染色法（Ｄｏｔ－ＩＧＳ）。以捕捉法Ｄｏｔ－ＩＧＳ检测３０份海南恶性疟病人全血，循环抗原阳性者２７份，阳性率为９０．０％；４０份其他寄生虫病人血样全为阴性。结果表明，捕捉法Ｄｏｔ－ＩＧＳ在敏感性和特异性方面都优于间接法Ｄｏｔ－ＩＧＳ和斑点酶联免疫吸附测定，是检测疟疾循环抗原的理想方法。
ot-immunogold staining(dot-IGS) method was established by using colloidal gold probes.27out of 30(90.0%) whole blood samples from patients of Hai'nan, were plasmodium falciparum circu-lating antigen positive. The results were negative when the immuned rabbit serum was replaced by nor-mal rabbit serum, and in samples from patients with other parasitic diseases,suggesting that capturedot-IGS was a sensitive and specific method for the detection of circulating antigen from patients'whole blood.
关键词
抗原
恶性疟原虫
染剂和染色法
Antigen Plasmodium falciparum Stains and staining
