以合成肽作抗原的酶联免疫吸附法诊断戊型肝炎病毒感染

Serodiagnosis of hepatitis E virus infection by an ELISA based on synthetic antigens

建立了用合成多肽抗原测定戊型肝炎病毒（ＨＥＶ）抗体的酶联免疫吸附法。合成多肽Ｅ３３（ｃ）（２μｇ／ｍｌ）、Ｅ１５（Ｍ）（１μｇ／ｍｌ）混合包被，测定了４８份ＨＥＶ抗体阳性血清，阳性符合率为９５．８％。４１份阴性血清均为阴性。此方法灵敏度高、特异性强、简便快速，适合临床应用。

n enzyme-linked immunosorbent assay(ELISA) for the detection of anti-NEV using syntheticpeptides was developed. 48 anti-HEV positive sera were tested with 2 peptides.The results showed that46 of them reacted with peptides of E33(C),E(15)M. None of 41 negative sera reacted with E33(C)andE15(M)It is a simple, fast,highly specific and sensitive method suitable for diagnosis ofhepatitis E infec-tion.