乙型肝炎患者血清抗HBs独特型抗体免疫复合物的检测

Stody on antiidiotypes against antiHBs-containing immune complexes in patients with hepatitis B

用聚乙二醇沉淀，胰蛋白酶处理乙型肝炎（乙肝）患者血清后，再用酶联免疫吸附法检测检样中的含抗ＨＢｓ独特型抗体免疫复合物（抗ＨＢｓ－Ａｂ＿２－ＩＣ）。结果发现，乙肝患者血清中ＩｇＧ、ＩｇＭ类抗ＨＢｓ－Ａｂ，－ＩＣ检出总阳性率分别为：急性乙型肝炎１４．５％（９／６２），慢性活动性肝炎２１９％（２８／１２８），慢性迁延性肝炎１２．５％（２／１６）。抗ＨＢｓ－Ａｂ＿２－ＩＣ阳性者的抗ＨＢｓ独特型抗体检出率８４．１％（３３／３９），且乙型肝炎病毒的血清学标志阳性率显著高于抗ＨＢｓ－Ａｂ＿２－ＩＣ阴性者。表明，乙肝患者体内存在抗ＨＢｓ－Ａｂ＿２－ＩＣ，抗ＨＢｓ独特型抗体（抗ＨＢｓ－Ａｂ＿２）可能通过与抗ＨＢｓ结合，削弱和影响抗ＨＢｓ中和、清除乙肝病毒抗原的作用。

he antiidiotypes against antiH Bs-containing Immune complexes (antiHBs-Ab_2-IC)in patients’serum with hepatitis B were determined by ELISA afrer precipitating IC by polyethylene glycol (PEG) and separating IC by trysin. The results showed that the positive rates of antiHBs-Ab_2-IC in the pa-tients with acute hepatitis B, chronically active hepatitis B and chronically procrastinated hepatitis B were 14.5%(9 / 62),21.9%(28/128)and 12.5%(2 / 16) respectivcly.In addition, antiidiotypes against antiHBs(antiHBs-Ab_2) and the marker of hepatitis B virus(HBV-m) could also be detected in most sera of patients with antiHBs-Ab_2-IC, suggesting that anti-HBs-Ab_2 may be combined with antiHBs and weaken the effects of antiHBs neutralizing HBV.