髓腔内电刺激联合活性人工骨修复骨缺损的实验研究

Experimental study of repairing of the rabbit radial defects with direct galvanic stimulation and BMP/DBX Composite graft.

目的探讨应用髓腔内电刺激与生物活性人工骨对兔桡骨缺损的修复作用。方法36只兔桡骨节段性骨/骨膜缺损模型随机分为三组,A组:去抗原牛松质骨(DBX)与骨形态发生蛋白(BMP)复合物植入缺损区,应用低压直流电刺激,B组:单纯BMP/DBX复合物植入,C组:单纯DBX植入。分别于2、5、9周取材,检测碱性磷酸酶(ALP)含量、进行组织形态学及电镜观察。结果较B、C组:A组术后2周,ALP含量明显升高,成骨细胞增生、血管生成活跃,9周骨缺损已由新生骨桥接,有骨改建进行。骨超微结构正常。结论联合应用电刺激与BMP复合去抗原松质骨修复兔桡骨缺损,作用明显。

Objective To explore the influence of low constant direct current (LCDC) in the medullary cavity with BMP/DBX Composite graft on repairing of rabbit radial defects. Methods The rabbits whose bilateral radius were the models of the segmental osteoperiosteum defect were divided into 3 groups, Group A: the defects with BMP/DBX Composite graft were stimulated by LCDC; Group B: the defects were only filled with BMP/ DBX Composite graft; Group C: the defects were only filled with DBX. At week 2,5 and 9, 4 animals from each group were put into death, and the healing was studied with radiology, histology & morphology, electron microscope and the contents of the and Alkaline Phosphatase (ALP). Results Compared with Group B and C: the healing of Group A was best. The contents of ALP were increasing obviously at week 2 (P<0.01), osteoblasts cells and growth of capillary showed higher vitality. In the mean time, bony bridging and bony remodeling took place. At week 9, bony ultrastructures were normal. Conclusion LCDC with BMP/DBX Composite graft is a better manner to repair the bone defect.