摘要
应用改良的一步法ＲＮＡ提取方法分离慢性粒细胞白血病（慢粒）患者外周血或骨髓标本总ＲＮＡ，以慢粒急性红白血病变细胞株为阳性对照，急件早幼粒细胞性白血病细胞株为阴性对照，建立逆转录／套式聚合酶链反应（ＲＴ，套式ＰＣＲ）检测断裂点簇集区一ａｂｌ融合基因。第一次ＰＣＲ的灵敏度为１：１０￣４水平，第二次ＰＣＲ后灵敏度提高至１：１０￣５水平。特异性扩增产物经用地高辛素标记寡核苷酸探针Ｓｏｕｔｈｅｒｎ杂交得到证实。
sing the modified single一step method to extract RNA from the peripheral blood or bone marrow of the patients with chronic mveloid leukemia. the author established RT/PCR and nested PCR to de-tect bcr一abl fused gene with the positive control K562 cell line and negative control HL一60 cell lineThe sensitivity of the first round of PCR is 1:10￣4 and it increases to 1:10￣5 after the second round ofPCR The special products have been confirmed by the Southern blot hybridization with Digoxigene la-beled oligonucleotide probe.
