摘要
报告用高效液用色谱（ＨＰＬＣ）测定孕妇尿内总雌三醇（Ｅ＿３）的方法。尿标本在酸水解后用乙醚提取Ｅ＿２，蒸干乙醚，残留物用流动相（甲醇／乙腈／水＝４０／１７／４３）重组。标本成分Ｃ＿８柱分离，以２８５ｎｍ波长激发，在６１０ｎｍ测定Ｅ＿３的自然荧光。本法批内ＣＶ为１２％～１．５％（ｘ＝３２．７～１２８．２ｕｍｏｌ／Ｌ），批间ＣＶ别为２．３％和４０％（分别是３８．４和９８．９ｕｍｏｌ／Ｌ）。本法在４．３３～１３８．７ｕｍｏｌ／Ｌ（１２５～４０．００ｍｇ／Ｌ）间呈线性，最低检出１．９ｕｍｏｌ／Ｌ（０．５２ｍｇ／Ｌ，信号／噪声比为３）。
e reported method for determining total estriol(E_3)in pregnancy urine by high preformance liq-uid chromatography(HPLC)With fluorescence detection E,was extracted by ethyl ether acid hvdrolysis;the extract evdporated,and the residue resoived with mobile phase(methanol/acetonitrile/water=40/17/43)before iniection into chromategraphy Sample components are sepa-rated with C8 coiumn and isocratiealy eluted.The,natural fluorescence of eluted estriol is measured by fiuorescence detector with excitation Wave length at 285nm and emission wave length at 610 nm The within一run CVs were 1.2%. 1. 4% aid 1.5% for32.68,89.16 and 128.19 umol/L of E_3 respectively;between一run CV was 2.4%for 38、37 umol/L and 4.0%for 98.9 1umol/L This method was linear in range of 4.33 to 138.7umol/L(125～40mg/L)The detection limit was 1.8umol/L(0.52mg/L,sig-nal/noise=3)。
