摘要
用特异性单克隆抗体,以免疫酶联法测定正常人及95例糖尿病患者尿液IgG_4,同时用放射免疫法测定尿中总IgG,结果糖尿病患者尿液中IgG_4水平高于正常对照组(P<0.01),其增高程度与白蛋白尿程度关系密切,而总IgG于临床蛋白尿阶段才明显增高。提示尿液IgG_4是反映肾小球损害程度较敏感的指标。
sing ELISA employing anti-IgG_4 monoclonal antibody,the levels of urinary IgG_4 was detected in95 diabetic patients and 2 9 normal controls.Urinary IgG_4 values were significantly higher in diabeticsubjects in comparison with controls (P<0.01).The increased urinary IgG_4 excretion correlated signifi-cantly with the degree of albuminuria。In the meantime,urinary total IgG was also determin ed byRIA,Urinary total IgG excretion increased significantly in clinical diabetic nephropathy,Urinary IgG_4could be a sensitive parameter to characterize incipient glomerular impairment。
关键词
糖尿病
肾病
单克隆抗体
免疫球蛋白G4
尿
Diabetic nephropathies Antibodies, monoclonnal Immunoglobulin G_4