用单克隆抗体定量测定糖尿病患者尿IgG_4

ELISA employing monoclonal antibody of rurinary IgG_4 in diabetic patients

用特异性单克隆抗体，以免疫酶联法测定正常人及９５例糖尿病患者尿液ＩｇＧ_４，同时用放射免疫法测定尿中总ＩｇＧ，结果糖尿病患者尿液中ＩｇＧ_４水平高于正常对照组（Ｐ＜０．０１），其增高程度与白蛋白尿程度关系密切，而总ＩｇＧ于临床蛋白尿阶段才明显增高。提示尿液ＩｇＧ_４是反映肾小球损害程度较敏感的指标。

sing ELISA employing anti-IgG_4 monoclonal antibody,the levels of urinary IgG_4 was detected in95 diabetic patients and 2 9 normal controls.Urinary IgG_4 values were significantly higher in diabeticsubjects in comparison with controls (P<0.01).The increased urinary IgG_4 excretion correlated signifi-cantly with the degree of albuminuria。In the meantime,urinary total IgG was also determin ed byRIA,Urinary total IgG excretion increased significantly in clinical diabetic nephropathy,Urinary IgG_4could be a sensitive parameter to characterize incipient glomerular impairment。