摘要
报道了简便、快速、准确的微量红细胞己糖激酶连续监测法。己糖激酶对葡萄糖的米氏常数为１．８×１０￣（－４）ｍｏ１／Ｌ。指示酶６－磷酸葡萄糖脱氢酶的最适浓度为２０Ｕ／ｍｌ。最适ｐＨ为８．２５。延迟时间为６０秒，线性反应时间从６０秒至１２分钟。线性范围为０～３６．０Ｕ／ｇ Ｈｂ。批内ｒｖ５．１５％，批间ＣＶ６．８９％。天津地区健康成人红细胞己糖激酶活性参考范围为０．７５～１．７１Ｕ／ｇＨｂ，脐血为０．９～１．５Ｕ／ｇＨｂ，低底物浓度百分比为（５５．３±４．３）％。
he authors reported a simple,rapidaccurate and feasible method for continrous monitoring ofhexokinase(HK)in red cells.Km of HK to glucose was 1.8 × 10￣(-4)mo1/L.The most appropriate con-centration of indicating enzyme G-6PD was 20U/ml and the most appropriate pH was 8.25.Delay timewas 60 seconds。The time of linear reaction lasted from 60 seconds to 12 minutes,The linear range was0～36U/g Hb;The coefficient of variation(CV)was 5.15%intra assay and 6.89%inter-assay.Thereference value in red cell of mormal aduIts in Tianjin area was 0.79～1.71U/g Hb and(55.3+4.3)%in low substrate concentrated condition.The value in umbilicus was 0.9～1.45U/g Hb blood.
